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[Effects of hexamethylene bisacetamide on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1].
- Source :
-
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology [Zhonghua Kou Qiang Yi Xue Za Zhi] 2002 Mar; Vol. 37 (2), pp. 120-2. - Publication Year :
- 2002
-
Abstract
- Objective: To investigate the effects of hexamethylene bisacetamide (HMBA) on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1.<br />Methods: MEC-1 was induced 72 hours by 0.002 mol/L HMBA in vitro. The morphologic observation and the expression of PCNA, bcl-2, bax protein and bcl-2 mRNA were determined by microscope, immunohistochemistry, in situ hybridization and image analysis.<br />Results: The expression of bax, bcl-2 protein and bcl-2 mRNA were higher than control group (P < 0.01). The related genes of cell proliferating PCNA were decreased in control group.<br />Conclusions: HMBA could inhibit the MEC-1 cell proliferation by down-regulation of PCNA expression, it could also induce apoptosis effectively that might be through the way of up-regulation of bax and bcl-2 gene expression.
- Subjects :
- Carcinoma, Mucoepidermoid drug therapy
Carcinoma, Mucoepidermoid metabolism
Cell Differentiation drug effects
Cell Division drug effects
Gene Expression drug effects
Genes, bcl-2
Humans
Proliferating Cell Nuclear Antigen analysis
RNA, Messenger analysis
Tumor Cells, Cultured
Acetamides pharmacology
Antineoplastic Agents pharmacology
Apoptosis genetics
Carcinoma, Mucoepidermoid pathology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1002-0098
- Volume :
- 37
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
- Publication Type :
- Academic Journal
- Accession number :
- 12475424