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RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica.
- Source :
-
European journal of biochemistry [Eur J Biochem] 2002 Dec; Vol. 269 (24), pp. 6152-61. - Publication Year :
- 2002
-
Abstract
- An RBCC (RING finger, B-box, and coiled-coil) protein was identified that belongs to the superfamily of zinc-binding proteins and is specifically expressed in the gill of eel, Anguilla japonica. Euryhaline fishes such as eels can migrate between freshwater and seawater, which is considered to be accomplished by efficient remodeling of the architecture and function of the gill, a major osmoregulatory organ. To identify molecules involved in such adaptive changes, we performed differential display using mRNA preparations from freshwater and seawater eel gills and obtained an RBCC clone among several differentially expressed clones. The clone encoded a protein of 514 amino acid residues with structural features characteristic of the RBCC protein; we therefore named it eRBCC (e for eel). eRBCC mRNA was specifically expressed in the gills with a greater extent in the gills of freshwater eels. Immunohistochemistry revealed that the expression of eRBCC is confined to particular epithelial cells of the gills including freshwater-specific lamellar chloride cells. The RING finger of eRBCC was found to have a ubiquitin ligase activity, suggesting an important regulatory role of eRBCC in the remodeling of branchial cells.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Western
COS Cells
Cloning, Molecular
DNA, Complementary metabolism
Eels metabolism
Epithelial Cells
Gene Expression Profiling
Gills metabolism
Immunohistochemistry
Ligases metabolism
Microscopy, Fluorescence
Molecular Sequence Data
Protein Binding
Protein Structure, Tertiary
RNA, Messenger metabolism
Ribonucleases metabolism
Sequence Homology, Amino Acid
Time Factors
Tissue Distribution
Ubiquitin metabolism
Eels genetics
Ligases biosynthesis
Ligases chemistry
Zinc Fingers
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 269
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12473111
- Full Text :
- https://doi.org/10.1046/j.1432-1033.2002.03332.x