Back to Search
Start Over
Testing genotypic and phenotypic resistance in human immunodeficiency virus type 1 isolates of clade B and other clades from children failing antiretroviral therapy.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2002 Dec; Vol. 40 (12), pp. 4512-9. - Publication Year :
- 2002
-
Abstract
- The emergence of resistance to antiretroviral drugs is a major obstacle to the successful treatment of human immunodeficiency virus type 1 (HIV-1)-infected patients. In this work, we correlate clinical and virological trends such as viral load (VL) and CD4 counts to genotypic and phenotypic antiretroviral (ARV) resistance profiles of HIV-1 isolates from the B and non-B subtypes found in vertically infected children failing ARV therapy. Plasma samples were collected from 52 vertically HIV-1-infected children failing different ARV therapies. Samples underwent HIV-1 pol sequencing and phenotyping and were clustered into subtypes by phylogenetic analysis. Clinical data from each patient were analyzed together with the resistance (genotypic and phenotypic) data obtained. Thirty-five samples were from subtype B, 10 samples were non-B (subtypes A, C, and F), and 7 were mosaic samples. There was no significant difference concerning treatment data between B and non-B clades. Prevalence of known drug resistance mutations revealed slightly significant differences among B and non-B subtypes: L10I, 21 and 64%, K20R, 13 and 43%, M36I, 34 and 100%, L63P, 76 and 36%, A71V/T, 24 and 0%, and V77I, 32 and 0%, respectively, in the protease (0.0001 </= P </= 0.0886), and D67N, 38 and 8%, K70R, 33 and 0%, R211K, 49 and 85%, and K219Q/E, 31 and 0%, respectively, in the reverse transcriptase (0.0256 </= P </= 0.0704). Significant differences were found only in secondary resistance mutations and did not reflect significant phenotypic variation between clade B and non-B.
- Subjects :
- Adolescent
Anti-HIV Agents pharmacology
Child
Child, Preschool
Drug Therapy, Combination
Female
Genes, pol
Genotype
HIV Infections virology
HIV Protease genetics
HIV Protease Inhibitors pharmacology
HIV Reverse Transcriptase genetics
HIV-1 enzymology
HIV-1 genetics
Humans
Male
Microbial Sensitivity Tests methods
Mutation
Phenotype
Reverse Transcriptase Inhibitors pharmacology
Treatment Failure
Anti-HIV Agents therapeutic use
Drug Resistance, Viral
HIV Infections drug therapy
HIV Protease Inhibitors therapeutic use
HIV-1 classification
HIV-1 drug effects
Reverse Transcriptase Inhibitors therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 40
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12454144
- Full Text :
- https://doi.org/10.1128/JCM.40.12.4512-4519.2002