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Genomic fingerprinting and genotyping of Helicobacter pylori strains from patients with duodenal ulcer or gastric cancer from different geographic regions.
- Source :
-
Digestive diseases and sciences [Dig Dis Sci] 2002 Nov; Vol. 47 (11), pp. 2512-8. - Publication Year :
- 2002
-
Abstract
- Continuing attempts have been made to classify pathogenic strains within bacterial populations based on DNA fingerprints and to identify virulence factors in H. pylori. We studied 287 H. pylori isolates from patients with duodenal ulcer or gastric cancer from three different geographic regions. DNA fingerprints were generated using REP-PCR and analyzed by cluster analysis. The status of three candidate virulence factors-vacA polymorphism, cagA and iceA,-were examined by PCR amplification. Cluster analysis of the REP-PCR fingerprints showed clustering by geographic region but not by disease presentation. cagA was detected in 91.3% of the isolates. Differences in vacA subtypes were observed among the three geographic regions. There was no association between iceA subtypes and clinical outcome. We conclude that geographic differences among the H. pylori strains exist in single gene allelic variants as well as in the conserved noncoding regions such as REP sequences throughout the entire bacterial genome. We did not detect any association between disease presentation and H. pylori genotypes using either DNA fingerprinting or candidate single gene virulence factors.
- Subjects :
- Bacterial Proteins genetics
DNA, Bacterial genetics
Demography
Genotype
Helicobacter Infections epidemiology
Helicobacter pylori pathogenicity
Humans
Polymerase Chain Reaction methods
Repetitive Sequences, Nucleic Acid
Virulence
Antigens, Bacterial
DNA Fingerprinting
Duodenal Ulcer microbiology
Helicobacter Infections microbiology
Helicobacter pylori genetics
Stomach Neoplasms microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0163-2116
- Volume :
- 47
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Digestive diseases and sciences
- Publication Type :
- Academic Journal
- Accession number :
- 12452388
- Full Text :
- https://doi.org/10.1023/a:1020564126960