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Redundancy in tumor necrosis factor (TNF) and lymphotoxin (LT) signaling in vivo: mice with inactivation of the entire TNF/LT locus versus single-knockout mice.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2002 Dec; Vol. 22 (24), pp. 8626-34. - Publication Year :
- 2002
-
Abstract
- Homologous genes and gene products often have redundant physiological functions. Members of the tumor necrosis factor (TNF) family of cytokines can signal activation, proliferation, differentiation, costimulation, inhibition, or cell death, depending on the type and status of the target cell. TNF, lymphotoxin alpha (LTalpha), and LTbeta form a subfamily of a larger family of TNF-related ligands with their genes being linked within a compact 12-kb cluster inside the major histocompatibility complex locus. Singly TNF-, LTalpha-, and LTbeta-deficient mice share several phenotypic features, suggesting that TNF/LT signaling pathways may regulate overlapping sets of target genes. In order to directly address the issue of redundancy of TNF/LT signaling, we used the Cre-loxP recombination system to create mice with a deletion of the entire TNF/LT locus. Mice with a triple LTbeta/TNF/LTalpha deficiency essentially manifest a combination of LT and TNF single-knockout phenotypes, except for microarchitecture of the spleen, where the disorder of lymphoid cell positioning and functional T- and B-cell compartmentalization is severer than that found in TNF or LT single-knockout mice. Thus, our data support the notion that TNF and LT have largely nonredundant functions in vivo.
- Subjects :
- Animals
B-Lymphocytes physiology
Gene Targeting
Leukocytes metabolism
Lymphoid Tissue cytology
Lymphoid Tissue metabolism
Lymphoid Tissue pathology
Mice
Mice, Knockout
Mice, Mutant Strains
Multigene Family
Spleen cytology
Spleen metabolism
T-Lymphocytes physiology
Gene Expression Regulation
Lymphotoxin-alpha metabolism
Signal Transduction physiology
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0270-7306
- Volume :
- 22
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 12446781
- Full Text :
- https://doi.org/10.1128/MCB.22.24.8626-8634.2002