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Stat3-dependent induction of BATF in M1 mouse myeloid leukemia cells.
- Source :
-
Oncogene [Oncogene] 2002 Nov 21; Vol. 21 (53), pp. 8186-91. - Publication Year :
- 2002
-
Abstract
- Stat3 mediates cellular responses associated with proliferation, survival and differentiation, but the mechanisms underlying the diverse effects of this signaling molecule remain unknown. M1 mouse myeloid leukemia cells arrest growth and differentiate into macrophages following treatment with interleukin 6 (IL-6) or leukemia inhibitory factor (LIF), and recent studies have shown that Stat3 plays a central role in this process. Utilizing representational difference analysis, we demonstrate that expression of the mouse BATF gene is upregulated as an early response to IL-6/LIF stimulation and Stat3 activation in this cell system. Immunoblots using antibodies to BATF detected an increase in BATF protein in response to LIF/IL-6 stimulation. BATF is a member of the AP-1 family of basic leucine zipper transcription factors and functions to inhibit the transcriptional and biological functions of AP-1 activity in mammalian cells. BATF forms complexes with c-Jun in M1 cells and forced expression of BATF in the absence of Stat3 signaling results in a reduced rate of cellular growth. These results indicate that Stat3 mediates cellular growth by modulating AP-1 activity through the induction of BATF.
- Subjects :
- Animals
Basic-Leucine Zipper Transcription Factors
Cell Division physiology
DNA-Binding Proteins genetics
Enzyme Inhibitors pharmacology
Flavonoids pharmacology
Growth Inhibitors pharmacology
Interleukin-6 pharmacology
Leucine Zippers
Leukemia Inhibitory Factor
Lymphokines pharmacology
MAP Kinase Signaling System drug effects
Mice
Neoplasm Proteins genetics
RNA, Messenger biosynthesis
RNA, Messenger genetics
RNA, Neoplasm biosynthesis
RNA, Neoplasm genetics
Recombinant Fusion Proteins physiology
STAT3 Transcription Factor
Trans-Activators genetics
Transcription Factors genetics
Transfection
Tumor Cells, Cultured metabolism
DNA-Binding Proteins physiology
Gene Expression Regulation, Leukemic physiology
Leukemia, Myeloid pathology
Neoplasm Proteins biosynthesis
Trans-Activators physiology
Transcription Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0950-9232
- Volume :
- 21
- Issue :
- 53
- Database :
- MEDLINE
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 12444555
- Full Text :
- https://doi.org/10.1038/sj.onc.1205918