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PMA decreases the proliferation of retinal cells in vitro: the involvement of acetylcholine and BDNF.
- Source :
-
Neurochemistry international [Neurochem Int] 2003 Jan; Vol. 42 (1), pp. 73-80. - Publication Year :
- 2003
-
Abstract
- Protein kinase C (PKC) is involved in several cell events including proliferation, survival and differentiation. The aim of this work was to investigate the role of PKC activation on retinal cells proliferation. We demonstrated that PKC activation by phorbol 12-myristate 13-acetate (PMA), a tumor promoter phorbol ester, is able to decrease retinal cells proliferation. This effect was mediated by M1 receptors and dependent on intracellular Ca(2+) increase, tyrosine kinase activity, phosphatidylinositol 3-kinase activity, polypeptide secretion and activation of TrkB receptors. The effect of PMA was not via activation of mitogen-activated protein (MAP) kinase. Carbamylcholine and brain derived neurotrophic factor were both able to decrease retinal cells proliferation to the same level as PMA did. Our results suggest that PKC activation leads to a decrease in retinal cells proliferation through the release of acetylcholine and brain derived neurotrophic factor in the culture, and activation of M1 and TrkB receptors, respectively.
- Subjects :
- Alkaloids
Amacrine Cells drug effects
Animals
Atropine pharmacology
Benzophenanthridines
Calcium pharmacology
Cell Division drug effects
Cells, Cultured drug effects
Dose-Response Relationship, Drug
Enzyme Activation drug effects
Enzyme Inhibitors pharmacology
Eye Proteins drug effects
Isoenzymes drug effects
Isoenzymes metabolism
MAP Kinase Signaling System drug effects
Muscarinic Agonists pharmacology
Muscarinic Antagonists pharmacology
Oxotremorine pharmacology
Phenanthridines pharmacology
Pirenzepine pharmacology
Protein Kinase C drug effects
Rats
Receptor, Muscarinic M1
Receptors, Muscarinic physiology
Retina cytology
Signal Transduction drug effects
Acetylcholine physiology
Brain-Derived Neurotrophic Factor pharmacology
Carbachol pharmacology
Eye Proteins metabolism
Growth Inhibitors pharmacology
Pirenzepine analogs & derivatives
Protein Kinase C metabolism
Receptors, Muscarinic drug effects
Retina drug effects
Tetradecanoylphorbol Acetate pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0197-0186
- Volume :
- 42
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neurochemistry international
- Publication Type :
- Academic Journal
- Accession number :
- 12441170
- Full Text :
- https://doi.org/10.1016/s0197-0186(02)00059-1