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Dorsal-ventral patterning of the spinal cord requires Gli3 transcriptional repressor activity.
- Source :
-
Genes & development [Genes Dev] 2002 Nov 15; Vol. 16 (22), pp. 2865-78. - Publication Year :
- 2002
-
Abstract
- Sonic hedgehog (Shh) plays a critical role in organizing cell pattern in the developing spinal cord. Gli proteins are thought to mediate Shh signaling, but their role in directing neural tube patterning remains unclear. Here we identify a role for Gli3 transcriptional repressor activity in patterning the intermediate region of the spinal cord that complements the requirement for Gli2 in ventral regions. Moreover, blocking all Gli responses results in a complete dorsalization of ventral spinal cord, indicating that in addition to the specific roles of Gli2 and Gli3 in the neural tube, there is functional redundancy between Gli proteins. Finally, analysis of Shh/Gli3 compound mutant mice substantiates the idea that ventral patterning may involve a mechanism independent, or parallel, to graded Shh signaling. However, even in the absence of graded Shh signaling, Gli3 is required for the dorsal-ventral patterning of the intermediate neural tube. Together these data raise the possibility that Gli proteins act as common mediators integrating Shh signals, and other sources of positional information, to control patterning throughout the ventral neural tube.
- Subjects :
- Animals
Chick Embryo
DNA-Binding Proteins genetics
Embryonic Induction
Hedgehog Proteins
Homeodomain Proteins metabolism
Humans
Kruppel-Like Transcription Factors
Mice
Mice, Mutant Strains
Nervous System embryology
Repressor Proteins genetics
Repressor Proteins metabolism
Spinal Cord metabolism
Spinal Cord pathology
Stem Cells physiology
Trans-Activators genetics
Trans-Activators metabolism
Transcription Factors genetics
Transcription, Genetic
Zinc Finger Protein Gli3
Body Patterning physiology
DNA-Binding Proteins metabolism
Nerve Tissue Proteins
Spinal Cord embryology
Transcription Factors metabolism
Xenopus Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0890-9369
- Volume :
- 16
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Genes & development
- Publication Type :
- Academic Journal
- Accession number :
- 12435629
- Full Text :
- https://doi.org/10.1101/gad.243402