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B lymphocyte memory: role of stromal cell complement and FcgammaRIIB receptors.
- Source :
-
The Journal of experimental medicine [J Exp Med] 2002 Nov 04; Vol. 196 (9), pp. 1189-99. - Publication Year :
- 2002
-
Abstract
- To dissect the influence of CD21/CD35 and FcgammaRIIB in antigen retention and humoral memory, we used an adoptive transfer model in which antigen-primed B and T lymphocytes were given to sublethally irradiated wild-type mice or mice deficient in CD21/CD35 (Cr2(-/-)) or FcgammaRIIB receptors (FcgammaRIIB(-/-)). Cr2(-/-) chimeras showed impaired memory as characterized by a decrease in antibody titer, reduced frequency of antibody secreting cells, an absence of affinity maturation, and significantly reduced recall response. The impaired memory in Cr2(-/-) chimeras corresponded with the reduced frequency of antigen-specific memory B cells. Interestingly, FcgammaRIIB(-/-) chimeras showed a differential phenotype with impaired splenic but normal bone marrow responses. These data suggest that CD21/CD35 on stroma, including follicular dendritic cells, is critical to the maintenance of long-term B lymphocyte memory.
- Subjects :
- Animals
Antibodies blood
Haptens
Hemocyanins immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Time Factors
Antigens, CD immunology
B-Lymphocytes immunology
Immunologic Memory immunology
Receptors, Complement 3b immunology
Receptors, Complement 3d immunology
Receptors, IgG immunology
Stromal Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 196
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 12417629
- Full Text :
- https://doi.org/10.1084/jem.20021110