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Activation of P2Y2 purinoceptor inhibits the activity of the Na+/K+-ATPase in HeLa cells.
- Source :
-
Cellular signalling [Cell Signal] 2003 Jan; Vol. 15 (1), pp. 115-21. - Publication Year :
- 2003
-
Abstract
- The role of ATP on regulation of the Na(+)/K(+)-ATPase activity in the human cancerous HeLa cells was investigated. HeLa cells stimulated with increasing ATP concentrations showed a dose-dependent inhibition of the Na(+)/K(+)-ATPase activity. These effects were also obtained by UTP. ATP and UTP provoked a rise in intracellular calcium concentration ([Ca(2+)](i)) persisting for at least 4 min. The inhibitor of phospholipase C, U73122, blocked the elevation of [Ca(2+)](i) provoked by ATP/UTP. The expression of mRNA for P2Y2 and P2Y6 receptors was demonstrated by RT-PCR. ATP/UTP activated PKC-alpha, -betaI and -epsilon isoforms, but not PKC-delta and -zeta. The inhibition of the Na(+)/K(+)-ATPase activity by ATP/UTP was blocked by Gö6976, a specific inhibitor of the calcium-dependent PKCs. In conclusion, our results suggest that ATP/UTP modulate Na(+)/K(+)-ATPase activity in HeLa cells through the P2Y2 purinoceptor via calcium mobilisation and activation of calcium-dependent PKCs.<br /> (Copyright 2002 Elsevier Science Inc.)
- Subjects :
- Calcium metabolism
Dose-Response Relationship, Drug
HeLa Cells
Humans
Kinetics
Protein Kinase C physiology
RNA, Messenger biosynthesis
Receptors, Purinergic P2Y2
Signal Transduction
Sodium-Potassium-Exchanging ATPase genetics
Adenosine Triphosphate pharmacology
Receptors, Purinergic P2 metabolism
Sodium-Potassium-Exchanging ATPase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0898-6568
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular signalling
- Publication Type :
- Academic Journal
- Accession number :
- 12401526
- Full Text :
- https://doi.org/10.1016/s0898-6568(02)00062-1