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In the immune synapse, ZAP-70 controls T cell polarization and recruitment of signaling proteins but not formation of the synaptic pattern.
- Source :
-
Immunity [Immunity] 2002 Oct; Vol. 17 (4), pp. 389-99. - Publication Year :
- 2002
-
Abstract
- Recognition by T cells of their ligands at the surface of antigen-presenting cells (APCs) leads to T cell activation, polarization of the T cell toward the APC, and formation of an immune synapse. Using ZAP-70-deficient T cells expressing zeta-GFP, we show that ZAP-70 signaling drives the TCR-dependent reorientation of the microtubule-organizing center thus leading to relocation of a zeta-GFP(+) intracellular compartment close to the APC. ZAP-70 is also necessary to supply the synapse with the signaling molecules PKC-theta and LAT. In contrast, ZAP-70 is not required for clustering of zeta-GFP and CD2 or exclusion of CD45 and CD43 from the synapse. These data show that ZAP-70-dependent signaling is required for formation of a functional immune synapse.
- Subjects :
- Antigen-Presenting Cells immunology
Antigen-Presenting Cells physiology
CD2 Antigens metabolism
Carrier Proteins metabolism
Cell Communication
Cell Line
Cell Polarity
Green Fluorescent Proteins
Humans
Intercellular Junctions physiology
Isoenzymes metabolism
Jurkat Cells
Leukocyte Common Antigens metabolism
Leukosialin
Luminescent Proteins metabolism
Lymphocyte Activation
Membrane Proteins metabolism
Microtubule-Organizing Center immunology
Microtubule-Organizing Center physiology
Phosphoproteins metabolism
Protein Kinase C metabolism
Protein Kinase C-theta
Receptors, Antigen, T-Cell metabolism
Recombinant Fusion Proteins metabolism
Sialoglycoproteins metabolism
Signal Transduction
ZAP-70 Protein-Tyrosine Kinase
Adaptor Proteins, Signal Transducing
Antigens, CD
Protein-Tyrosine Kinases physiology
T-Lymphocytes immunology
T-Lymphocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1074-7613
- Volume :
- 17
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Immunity
- Publication Type :
- Academic Journal
- Accession number :
- 12387734
- Full Text :
- https://doi.org/10.1016/s1074-7613(02)00421-1