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The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.

Authors :
Przetakiewicz J
Nadolska-Orczyk A
Orczyk W
Source :
Cellular & molecular biology letters [Cell Mol Biol Lett] 2002; Vol. 7 (2B), pp. 671-6.
Publication Year :
2002

Abstract

Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.

Details

Language :
English
ISSN :
1425-8153
Volume :
7
Issue :
2B
Database :
MEDLINE
Journal :
Cellular & molecular biology letters
Publication Type :
Academic Journal
Accession number :
12378226