Back to Search
Start Over
Molecular cloning and characterization of a novel human Rab ( Rab2B) gene.
- Source :
-
Journal of human genetics [J Hum Genet] 2002; Vol. 47 (10), pp. 548-51. - Publication Year :
- 2002
-
Abstract
- Rab proteins are small-molecular-weight guanosine triphosphatases (GTPases) that control vesicular traffic in eukaryotic cells. The small GTPase Rab2 is a resident of pre-Golgi intermediates and is required for protein transport from the endoplasmic reticulum to the Golgi complex. We identified a novel human Rab (Rab2B) gene that was 2312 bp in length and encoded a protein of 216 amino acid residues. The protein shared high homology with mouse Rab2 (identity 83%, similarity 91%). The expression pattern of the human Rab2B gene showed that there is a transcript in kidney, prostate, lung, liver, thymus, colon, pancreas, and skeletal muscle, and low levels in placenta, whereas specific bands of the transcript could not be detected in heart, brain, spleen, testis, ovary, small intestine, and leukocyte. Overexpression has been observed in colon adenocarcinoma CX-1. The Rab2B gene consists of nine exons and eight introns and is mapped to chromosome 14q11.1-14q11.2 by bioinformatics analysis.
- Subjects :
- Aged
Amino Acid Sequence
Animals
Blotting, Northern
Chromosome Mapping
Chromosomes, Human, Pair 14 genetics
Cloning, Molecular
DNA, Complementary
Exons
Female
Gene Expression
Humans
Introns
Male
Mice
Middle Aged
Molecular Sequence Data
RNA, Messenger metabolism
Sequence Homology, Amino Acid
Tissue Distribution
rab2 GTP-Binding Protein metabolism
rab2 GTP-Binding Protein genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1434-5161
- Volume :
- 47
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of human genetics
- Publication Type :
- Academic Journal
- Accession number :
- 12376746
- Full Text :
- https://doi.org/10.1007/s100380200083