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Co-immunization with M-CSFR and mM-CSF DNA vaccines is better than M-CSFR-mM-CSF fusion DNA vaccine.
- Source :
-
Haematologica [Haematologica] 2002 Oct; Vol. 87 (10), pp. 1087-94. - Publication Year :
- 2002
-
Abstract
- Background and Objectives: DNA vaccine against macrophage colony-stimulating factor receptor (M-CSFR) has shown both protective and therapeutic effects. In this study, we explore the possibility of using DNA vaccines against both M-CSFR and membrane-bound macrophage colony-stimulating factor (mM-CSF) to achieve better effects.<br />Design and Methods: Three plasmids were constructed by inserting either extracellular and transmembrane region of mM-CSF (pM), or extracellular region of M-CSFR (pR), or extracellular region of M-CSFR linked with extracellular and transmembrane regions of mM-CSF by a (Gly Gly Ser)2 flexible linker (pF), into pcDNA3.1. A SP2/0 cell line stably expressing pF (SP2/0-F) was established to evaluate humoral and cytotoxic immune responses as well as therapeutic and preventive effects induced by pM, pR, pF or pM+pR vaccination in BALB/c mice. The mechanisms of these vaccinations were also studied by monitoring the release of interleukin (IL)-4 and interferon (IFN)-g by splenocytes upon activation.<br />Results: Vaccination against two epitopes had better effects than against a single epitope while vaccination by pM+pR had the greatest effects on inducing humoral and cytotoxic immune responses, prolonging survival of mice challenged with SP2/0-F, and inducing IL-4 and IFN-g release by splenocytes.<br />Interpretation and Conclusions: Our results suggest that co-immunization of M-CSFR and mM-CSF DNA vaccines is better than M-CSFR-mM-CSF fusion DNA vaccine.
- Subjects :
- Animals
Blotting, Western
COS Cells
Cytokines metabolism
Enzyme-Linked Immunosorbent Assay
Epitopes
Interferon-gamma metabolism
Interleukin-4 metabolism
Mice
Mice, Inbred BALB C
Neoplasm Transplantation
Neoplasms drug therapy
Plasmids metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spleen cytology
Time Factors
Transfection
Tumor Cells, Cultured
Cancer Vaccines
Cell Membrane metabolism
Receptor, Macrophage Colony-Stimulating Factor therapeutic use
Vaccines, DNA
Subjects
Details
- Language :
- English
- ISSN :
- 0390-6078
- Volume :
- 87
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Haematologica
- Publication Type :
- Academic Journal
- Accession number :
- 12368165