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The dose-response relationship for ultraviolet-light-induced mutations at the hypoxanthine-guanine phosphoribosyltransferase locus in Chinese hamster ovary cells.
- Source :
-
Somatic cell genetics [Somatic Cell Genet] 1975 Oct; Vol. 1 (4), pp. 383-9. - Publication Year :
- 1975
-
Abstract
- Exposure of Chinese hamster ovary (CHO) cells clone K1BH4 to ultraviolet (UV) light at doses up to 86 ergs/mm2 did not significantly reduce cell survival, but UV doses of 86-648 ergs/mm2 produced an exponential cell killing. Observed mutation frequency ro 6-thioguannine resistance induced by UV increases approximately in proportion to increasing doses up to 260 ergs/mm2 in a range of 5-648 ergs/mm2 examined. The pooled data of mutation frequency f(X) as a function of dose X from 0-260 ergs/mm2 is adequately described by f(X)=10(-6) (13.6 + 2.04 X). That the UV-induced mutations to 6-thioguanine resistance affects the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus is supported by the observation that all randomly isolated drug-resistant colonies contained highly reduced or undetectable HGPRT activity.
Details
- Language :
- English
- ISSN :
- 0098-0366
- Volume :
- 1
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Somatic cell genetics
- Publication Type :
- Academic Journal
- Accession number :
- 1235913
- Full Text :
- https://doi.org/10.1007/BF01538669