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Tissue-engineered composites for the repair of large osteochondral defects.
- Source :
-
Arthritis and rheumatism [Arthritis Rheum] 2002 Sep; Vol. 46 (9), pp. 2524-34. - Publication Year :
- 2002
-
Abstract
- Objective: To test the hypothesis that engineered cartilage can provide a mechanically functional template capable of undergoing orderly remodeling during the repair of large osteochondral defects in adult rabbits, as assessed by quantitative structural and functional methods.<br />Methods: Engineered cartilage generated in vitro from chondrocytes cultured on a biodegradable scaffold was sutured to a subchondral support and the resulting composite press-fitted into a 7-mm long, 5-mm wide, 5-mm deep osteochondral defect in a rabbit knee joint. Defects left empty (group 1) or treated with cell-free composites (group 2) served as controls for defects treated with composites of engineered cartilage and the support, without or with adsorbed bone marrow (groups 3 and 4, respectively).<br />Results: Engineered cartilage withstood physiologic loading and remodeled over 6 months into osteochondral tissue with characteristic architectural features and physiologic Young's moduli. Composites integrated well with host bone in 90% of cases but did not integrate well with host cartilage. Structurally, 6-month repairs in groups 3 and 4 were superior to those in group 2 with respect to histologic score, cartilage thickness, and thickness uniformity, but were inferior to those in unoperated control tissue. At 6 months, Young's moduli in groups 2, 3, and 4 (0.68, 0.80, and 0.79 MPa, respectively) approached that in unoperated control tissue (0.84 MPa), whereas the corresponding modulus in group 1 (0.37 MPa) was significantly lower.<br />Conclusion: Composites of tissue-engineered cartilage and a subchondral support promote the orderly remodeling of large osteochondral defects in adult rabbits.
- Subjects :
- Animals
Bone Diseases pathology
Bone Diseases physiopathology
Bone Remodeling
Cartilage pathology
Cells, Cultured
Chondrocytes pathology
Chondrocytes physiology
Knee Joint pathology
Knee Joint physiopathology
Male
Rabbits
Weight-Bearing
Bioprosthesis
Bone Diseases surgery
Cartilage physiology
Knee Joint surgery
Tissue Engineering
Subjects
Details
- Language :
- English
- ISSN :
- 0004-3591
- Volume :
- 46
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Arthritis and rheumatism
- Publication Type :
- Academic Journal
- Accession number :
- 12355501
- Full Text :
- https://doi.org/10.1002/art.10493