Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis.

Authors :: Reischl U
Linde HJ
Lehn N
Landt O
Barratt K
Wellinghausen N
Source :: Journal of clinical microbiology [J Clin Microbiol] 2002 Oct; Vol. 40 (10), pp. 3814-7.
Publication Year :: 2002
Abstract: A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.

Subjects :: Computer Systems
Humans
Legionella pneumophila classification
Legionella pneumophila genetics
Nucleic Acid Denaturation
RNA, Ribosomal, 16S analysis
RNA, Ribosomal, 16S genetics
DNA, Bacterial analysis
Legionella pneumophila isolation & purification
Polymerase Chain Reaction methods

Full Text Access

Tools