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Coreceptor Switch of [MLV(SIVagm)] pseudotype vectors by V3-loop exchange.
- Source :
-
Virology [Virology] 2002 Sep 01; Vol. 300 (2), pp. 205-16. - Publication Year :
- 2002
-
Abstract
- Retroviral vectors derived from murine leukemia virus (MLV) have been pseudotyped with a variant of the envelope glycoprotein (Env) of nonpathogenic simian immunodeficiency virus from African green monkeys (SIVagm) to result in [MLV(SIVagm-wt)] vector particles. The variant env gene encodes a full-length surface envelope glycoprotein (SU) and a C-terminally truncated transmembrane protein (TM). To change the coreceptor usage of this vector from CCR5 to CXCR4, which is predominant on human CD4-positive lymphocytes, the putative V3-loop of SIVagm SU was replaced by that of the T cell tropic HIV-1 variant BH10. The resulting [MLV(SIVagm-X4)] vectors were shown to specifically transduce CD4/CXCR4-positive cell lines, demonstrating the equivalent function in cell entry and choice of coreceptor usage of the V3-loops of SIVagm and HIV-1. These modified vectors were able to transduce primary human lymphocytes and were resistant to neutralization by sera from HIV-1-infected individuals. The [MLV(SIVagm-X4)] pseudotype vector generated is thus a promising candidate vector, e.g., for in vivo gene therapy of HIV-1 infection.
- Subjects :
- Acquired Immunodeficiency Syndrome blood
Acquired Immunodeficiency Syndrome therapy
Amino Acid Sequence
Animals
Cell Line
Chlorocebus aethiops
Gene Products, env physiology
Genetic Therapy
HIV-1 physiology
Humans
Molecular Sequence Data
Simian Immunodeficiency Virus physiology
Virus Assembly
Gene Products, env chemistry
Genetic Vectors
HIV-1 chemistry
Leukemia Virus, Murine genetics
Receptors, CCR5 physiology
Receptors, CXCR4 physiology
Simian Immunodeficiency Virus chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 300
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 12350351
- Full Text :
- https://doi.org/10.1006/viro.2001.1565