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Effect of trimethylcolchicinic acid on the synthesis and excretion of proteoglycans in tissue culture.
- Source :
-
European journal of biochemistry [Eur J Biochem] 1975 Jan 15; Vol. 50 (3), pp. 563-9. - Publication Year :
- 1975
-
Abstract
- The action of trimethylcolchicinic acid on the synthesis and excretion of proteoglycans has been studied on the L cell strain. The incorporation of precursors has been measured, and proteoglycans produced in the culture medium have been extracted and their concentration determined. The mucopolysaccharide components have been studied by electrophoresis. Control cultures produce hyaluronic acid, dermatan sulfate and very low concentrations of chondroitin 4-sulphate or 6-sulphate. Cultures treated with trimethycolchicinic acid (4 mu g/ml) produce hyaluronic acid, very high concentrations of chondroitin 4-sulphate or 6-sulphate and only traces of dermatan sulphate. So, trimethylcolchicinic acid does not modify the synthesis of hyaluronic acid: it considerably increases the production of chondroitin 4-sulphate or 6-sulphate and inhibits the production of dermatan sulphate. Protein fraction of the proteoglycans is proportionally increased in treated cultures, but there is no marked difference between amino acid concentrations of proteoglycans extracted from control and treated cultures. A slight fall in the cystine concentrations was the only change in the amino acid content of proteoglycans extracted from treated cultures. A hypothesis to explain these results is discussed.
- Subjects :
- Amino Acids metabolism
Chondroitin metabolism
Colchicine pharmacology
Dermatan Sulfate metabolism
Electrophoresis, Starch Gel
Glucosamine metabolism
Glucosephosphates metabolism
Hexosamines metabolism
Hyaluronic Acid metabolism
L Cells metabolism
Methylation
Sulfates metabolism
Sulfur Radioisotopes
Colchicine analogs & derivatives
Glycosaminoglycans metabolism
L Cells drug effects
Proteoglycans metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 50
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 123198
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1975.tb09897.x