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The immunomodulatory actions of prostaglandin E2 on allergic airway responses in the rat.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2002 Oct 01; Vol. 169 (7), pp. 3963-9. - Publication Year :
- 2002
-
Abstract
- PGE(2) has been reported to inhibit allergen-induced airway responses in sensitized human subjects. The aim of this study was to investigate the mechanism of anti-inflammatory actions of PGE(2) in an animal model of allergic asthma. BN rats were sensitized to OVA using Bordetella pertussis as an adjuvant. One week later, an aerosol of OVA was administered. After a further week, animals were anesthetized with urethan, intubated, and subjected to measurements of pulmonary resistance (R(L)) for a period of 8 h after OVA challenge. PGE(2) (1 and 3 micro g in 100 micro l of saline) was administered by insufflation intratracheally 30 min before OVA challenge. The early response was inhibited by PGE(2) (3 micro g). The late response was inhibited by both PGE(2) (1 and 3 micro g). Bronchoalveolar lavage fluid from OVA-challenged rats showed eosinophilia and an increase in the number of cells expressing IL-4 and IL-5 mRNA. These responses were inhibited by PGE(2). Bronchoalveolar lavage fluid levels of cysteinyl-leukotrienes were elevated after OVA challenge and were reduced after PGE(2) to levels comparable with those of sham challenged animals. We conclude that PGE(2) is a potent anti-inflammatory agent that may act by reducing allergen-induced Th2 cell activation and cysteinyl-leukotriene synthesis in the rat.
- Subjects :
- Adjuvants, Immunologic administration & dosage
Administration, Inhalation
Aerosols
Airway Resistance drug effects
Airway Resistance immunology
Animals
Bronchoalveolar Lavage Fluid cytology
Bronchoalveolar Lavage Fluid immunology
Cysteine antagonists & inhibitors
Cysteine biosynthesis
Dinoprostone administration & dosage
Hypersensitivity, Delayed immunology
Hypersensitivity, Delayed physiopathology
Hypersensitivity, Immediate immunology
Hypersensitivity, Immediate physiopathology
Interferon-gamma biosynthesis
Interferon-gamma genetics
Interleukin-4 biosynthesis
Interleukin-4 genetics
Interleukin-5 biosynthesis
Interleukin-5 genetics
Intubation, Intratracheal
Leukocyte Count
Leukotrienes biosynthesis
Male
Ovalbumin administration & dosage
Ovalbumin immunology
Pulmonary Alveoli metabolism
RNA, Messenger biosynthesis
Rats
Rats, Inbred BN
Receptors, Prostaglandin E metabolism
Respiratory Hypersensitivity physiopathology
Adjuvants, Immunologic pharmacology
Dinoprostone pharmacology
Respiratory Hypersensitivity immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 169
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 12244197
- Full Text :
- https://doi.org/10.4049/jimmunol.169.7.3963