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Plasmalemma abscisic acid perception leads to RAB18 expression via phospholipase D activation in Arabidopsis suspension cells.
- Source :
-
Plant physiology [Plant Physiol] 2002 Sep; Vol. 130 (1), pp. 265-72. - Publication Year :
- 2002
-
Abstract
- Abscisic acid (ABA) plays a key role in the control of stomatal aperture by regulating ion channel activities and water exchanges across the plasma membrane of guard cells. Changes in cytoplasmic calcium content and activation of anion and outward-rectifying K(+) channels are among the earliest cellular responses to ABA in guard cells. In Arabidopsis suspension cells, we have demonstrated that outer plasmalemma perception of ABA triggered similar early events. Furthermore, a Ca(2+) influx and the activation of anion channels are part of the ABA-signaling pathway leading to the specific expression of RAB18. Here, we determine whether phospholipases are involved in ABA-induced RAB18 expression. Phospholipase C is not implicated in this ABA pathway. Using a transphosphatidylation reaction, we show that ABA plasmalemma perception results in a transient stimulation of phospholipase D (PLD) activity, which is necessary for RAB18 expression. Further experiments showed that PLD activation was unlikely to be regulated by heterotrimeric G proteins. We also observed that ABA-dependent stimulation of PLD was necessary for the activation of plasma anion current. However, when ABA activation of plasma anion channels was inhibited, the ABA-dependent activation of PLD was unchanged. Thus, we conclude that in Arabidopsis suspension cells, ABA stimulation of PLD acts upstream from anion channels in the transduction pathway leading to RAB18 expression.
- Subjects :
- Arabidopsis cytology
Arabidopsis enzymology
Cell Membrane drug effects
Cells, Cultured
Enzyme Activation drug effects
Gene Expression Regulation, Enzymologic drug effects
Gene Expression Regulation, Plant drug effects
Heterotrimeric GTP-Binding Proteins metabolism
Ion Channels drug effects
Membrane Potentials drug effects
Membrane Potentials physiology
Signal Transduction drug effects
Signal Transduction physiology
Substrate Specificity
Type C Phospholipases metabolism
Abscisic Acid pharmacology
Arabidopsis genetics
Arabidopsis Proteins genetics
Cell Membrane physiology
Phospholipase D metabolism
rab GTP-Binding Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0889
- Volume :
- 130
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 12226506
- Full Text :
- https://doi.org/10.1104/pp.004168