Back to Search
Start Over
Immunohistochemical analysis of lymphocyte subpopulations in cyclosporin A-induced gingival overgrowth.
- Source :
-
Journal of periodontology [J Periodontol] 2002 Aug; Vol. 73 (8), pp. 892-9. - Publication Year :
- 2002
-
Abstract
- Background: Cyclosporin A (CsA) is an immunosuppressive agent that is known to induce gingival overgrowth (GO). Pharmacological, genetic, immunologic, and inflammatory factors seem to be involved in the complex pathogenesis of drug-induced GO. Lymphocyte subpopulations in human gingival connective tissue have been implicated in the pathogenesis of inflammatory periodontal diseases. One purpose of this study was to quantify CD4, CD8-, CD57-, and epithelial membrane antigen (EMA)-positive cells in the gingiva of renal transplant recipients treated with CsA, and compare them to findings in healthy controls. A second aim was to correlate cell numbers with clinical findings.<br />Methods: The study included 19 kidney recipients who were taking CsA and had significant GO (CsAGO+), 13 recipients who were taking CsA but showed no GO (CsAGO-), and 14 systemically healthy individuals with gingivitis (C). Sections from gingival biopsies were incubated with monoclonal antibodies for CD4, CD8, EMA, and CD57, and then analyzed using the avidin-biotin complex method. In each specimen, the mononuclear cell types were quantified and their distribution was evaluated in 3 separate tissue zones: S = subepithelial connective tissue beneath the sulcular epithelium; O = subepithelial connective tissue beneath the oral epithelium; and M = middle connective tissue.<br />Results: There were no significant differences among the groups with respect to the numbers of CD4+ and CD8+ cells in each of the 3 zones (P >0.05). In zone S, the CsAGO+ group had significantly more EMA-positive cells than either the C or CsAGO- groups (P <0.05). There were significant differences among the groups regarding numbers of CD57+ (natural killer) cells in zone M, with the lowest cell numbers in the CsAGO+ patients (P<0.05).<br />Conclusions: The results showed that low numbers of natural killer cells are important in the expression of plaque-induced inflammatory changes in CsA-associated GO. It appears that these cells may influence the drug's ability to induce proliferative activity.
- Subjects :
- Adult
Analysis of Variance
Antibodies, Monoclonal
CD4-Positive T-Lymphocytes classification
CD57 Antigens analysis
CD8-Positive T-Lymphocytes classification
Connective Tissue drug effects
Connective Tissue pathology
Dental Plaque Index
Female
Gingiva drug effects
Gingiva pathology
Gingival Overgrowth chemically induced
Gingivitis pathology
Humans
Immunoenzyme Techniques
Immunohistochemistry
Kidney Transplantation pathology
Killer Cells, Natural classification
Lymphocyte Count
Male
Mucin-1 analysis
Periodontal Index
Plasma Cells classification
Statistics as Topic
Statistics, Nonparametric
Cyclosporine adverse effects
Gingival Overgrowth pathology
Immunosuppressive Agents adverse effects
Lymphocyte Subsets classification
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3492
- Volume :
- 73
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 12211499
- Full Text :
- https://doi.org/10.1902/jop.2002.73.8.892