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Reduction of CpG-induced arthritis by suppressive oligodeoxynucleotides.
- Source :
-
Arthritis and rheumatism [Arthritis Rheum] 2002 Aug; Vol. 46 (8), pp. 2219-24. - Publication Year :
- 2002
-
Abstract
- Objective: Bacterial DNA contains immunostimulatory CpG motifs that cause inflammation when injected into the knee joints of normal mice. We examined whether synthetic oligodeoxynucleotides (ODN) that suppress CpG-induced immune responses prevent CpG-induced arthritis.<br />Methods: CpG, suppressive, and/or control ODN were injected into the knees of BALB/c mice. Joint swelling and inflammation were evaluated by physical measurement, by histologic analysis of joint tissue, and by magnetic resonance imaging.<br />Results: Immunostimulatory CpG DNA induced local arthritis, characterized by swelling of the knee joints, the presence of inflammatory cell infiltrates, the perivascular accumulation of mononuclear cells, and hyperplasia of the synovial lining. Administering suppressive (but not control) ODN reduced the manifestations and severity of arthritis up to 80%.<br />Conclusion: Suppressive ODN may be useful for the prevention or treatment of arthritis induced by bacterial DNA.
- Subjects :
- Animals
Arthritis, Experimental immunology
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Injections, Intra-Articular
Knee Joint drug effects
Knee Joint metabolism
Knee Joint pathology
Magnetic Resonance Imaging
Mice
Mice, Inbred BALB C
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Arthritis, Experimental drug therapy
Arthritis, Reactive drug therapy
CpG Islands
Oligonucleotides therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 0004-3591
- Volume :
- 46
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Arthritis and rheumatism
- Publication Type :
- Academic Journal
- Accession number :
- 12209528
- Full Text :
- https://doi.org/10.1002/art.10423