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Detection of circulating hypodermin C: an antigen capture ELISA for diagnosis of cattle grub (Diptera: Oestridae) infestations.
- Source :
-
Veterinary parasitology [Vet Parasitol] 2002 Aug 30; Vol. 108 (1), pp. 85-94. - Publication Year :
- 2002
-
Abstract
- An antigen capture assay for the detection of circulating hypodermin C was developed for diagnosis of hypodermosis. A murine monoclonal antibody to recombinant hypodermin C was raised using rapid immunization and a one-step hybridization-cloning technique. A highly reactive, specific monoclonal antibody was tested using sera spiked with known quantities of purified, native hypodermin C or with recombinant hypodermin C. Sensitivity of 96.4% and specificity of 95.6% for the antigen capture assay was assessed using a panel of sera from animals unexposed to cattle grubs and from cattle with palpation proven cattle grub infestations. Data from this panel of sera was used to establish the cut-off OD for further testing. The kinetics of circulating hypodermin C was assessed using the assay in three groups of cattle artificially infested with 50, 100 or 200 first instar Hypoderma lineatum. Antigen was first detected approximately 6 weeks after infestation. The amount of antigen detected increased in each group of animals reaching peaks at different times in each group. Levels of antigen fell quickly following arrival of grubs at the back and completion of the molt to second instar.
- Subjects :
- Animals
Antibodies, Monoclonal biosynthesis
Antibodies, Monoclonal blood
Antigens immunology
Cattle
Cattle Diseases parasitology
Diptera metabolism
Enzyme-Linked Immunosorbent Assay methods
Hypodermyiasis diagnosis
Hypodermyiasis parasitology
Sensitivity and Specificity
Serine Endopeptidases immunology
Time Factors
Cattle Diseases diagnosis
Diptera immunology
Enzyme-Linked Immunosorbent Assay veterinary
Hypodermyiasis veterinary
Serine Endopeptidases blood
Subjects
Details
- Language :
- English
- ISSN :
- 0304-4017
- Volume :
- 108
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 12191902
- Full Text :
- https://doi.org/10.1016/s0304-4017(02)00179-6