The effects of chemical occlusion of vas deferens on the testicular function of dog: a preliminary study.

A new approach to rapid male sterilization was studies by giving a single injection of sclerosing chemical agents (10% silvernitrate, 3.6% Formaldehyde in ehanol, 5% potassium permagnate, 100% ethanol, and 3.6% Formaldehyde) directly into the vas deferens to determine whether blocking the vas has any adverse effects on the testicular function. 2 - Chemical occlusion of vas is quite effective in producing a block in the vas deferens of dogs. 3 - Ethanol injection in the vas deferens caused an atrophy of the testis. Extensive necrossis and exfoliation of the seminiferous elements were conspicuous. These changes increased in severity in 3.6% Formaldehyde in ethanol, 5% potassium permagnate, and 10% silver nitrate solution injected dogs. 4 - Decrease in the testicular contents of RNA, Protein and sialic acid, four weeks after vas occlusion were associated with degenerative changes of the spermatogenic elements. 5 - Testicular cholesterol and total lipids were increased following vas occlusion. 6 - Reduced sialic acid levels in the testis of vas occluded dogs indicates an inhibition of androgen production, which is further reflected in reduced leydig cell function. 7 - The role of vas deferens in determining the fate of a given testis is being discussed.