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Priming of human oral epithelial cells by interferon-gamma to secrete cytokines in response to lipopolysaccharides, lipoteichoic acids and peptidoglycans.
- Source :
-
Journal of medical microbiology [J Med Microbiol] 2002 Aug; Vol. 51 (8), pp. 626-634. - Publication Year :
- 2002
-
Abstract
- An earlier study reported that human gingival epithelial cells in primary culture and oral epithelial cell lines KB and HSC-2 cells were devoid of membrane CD14 (mCD14) and did not show enhanced production of interleukin (IL)-8 or granulocyte macrophage-colony stimulating factor (GM-CSF) upon stimulation with bacterial cell-surface components such as lipopolysaccharide (LPS), lipoteichoic acid (LTA), peptidoglycan (PGN) and synthetic muramyldipeptide (MDP) even in the presence of serum. The present study demonstrated that after treatment with interferon (IFN)-gamma for 3 days, these cells secreted IL-8 and GM-CSF in response to the bacterial components. Treatment with IFN-gamma enhanced Toll-like receptor (TLR) 2, TLR4, MD-2 and MyD88 mRNA expression as determined by reverse transcriptase PCR. Anti-TLR2 and anti-TLR4 monoclonal antibodies (MAbs) inhibited the IL-8 production induced by PGN and LTA as well as LPS, respectively, in IFN-gamma-primed oral epithelial cells, whereas neither MAb inhibited IL-8 production induced by MDP. These findings suggested that IFN-gamma primed oral epithelial cells to produce cytokines upon stimulation with various bacterial components by up-regulation of the TLR system.
- Subjects :
- Acetylmuramyl-Alanyl-Isoglutamine immunology
Adaptor Proteins, Signal Transducing
Antigens, Differentiation biosynthesis
Antigens, Differentiation genetics
Antigens, Surface biosynthesis
Antigens, Surface genetics
Child
Cytokines biosynthesis
Cytokines metabolism
Epithelial Cells drug effects
Epithelial Cells immunology
Epithelial Cells metabolism
Female
Gingiva drug effects
Gingiva metabolism
Granulocyte-Macrophage Colony-Stimulating Factor immunology
Granulocyte-Macrophage Colony-Stimulating Factor metabolism
Humans
Interferon-gamma pharmacology
Interleukin-8 immunology
Interleukin-8 metabolism
Lipopolysaccharides immunology
Lipopolysaccharides pharmacology
Lymphocyte Antigen 96
Membrane Glycoproteins biosynthesis
Membrane Glycoproteins genetics
Myeloid Differentiation Factor 88
Peptidoglycan immunology
Peptidoglycan pharmacology
RNA chemistry
RNA genetics
Receptors, Cell Surface biosynthesis
Receptors, Cell Surface genetics
Receptors, Immunologic biosynthesis
Receptors, Immunologic genetics
Reverse Transcriptase Polymerase Chain Reaction
Teichoic Acids immunology
Teichoic Acids pharmacology
Toll-Like Receptor 2
Toll-Like Receptor 4
Toll-Like Receptors
Adjuvants, Immunologic pharmacology
Cytokines immunology
Drosophila Proteins
Gingiva immunology
Interferon-gamma immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2615
- Volume :
- 51
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12171292
- Full Text :
- https://doi.org/10.1099/0022-1317-51-8-626