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Mitochondrial permeability transition induced by chemically generated singlet oxygen.
- Source :
-
Journal of bioenergetics and biomembranes [J Bioenerg Biomembr] 2002 Jun; Vol. 34 (3), pp. 157-63. - Publication Year :
- 2002
-
Abstract
- Pure singlet molecular oxygen (1O2) generated by thermal decomposition of the 3,3'-(1,4-naphthylidene) dipropionate endoperoxide (NDPO2), inhibited respiration of isolated rat liver mitochondria supported by NADH-linked substrates or succinate, but not by N,N,N,N-tetramehyl-p-phenylene-diamine (TMPD)/ascorbate. Under the latter conditions, mitochondria treated with 2.7 mM NDPO2 exhibited a decrease in transmembrane potential (deltapsi) in manner dependent on NDPO2 exposure time. This process was sensitive to the mitochondrial permeability transition inhibitors EGTA, dithiothreitol, ADP, and cyclosporin A. The presence of deuterium oxide (D2O), that increases 1O2 lifetime, significantly enhanced NDPO2-promoted mitochondrial pereabilization. In addition, NDPO2-induced mitochondrial permeabilization was accompanied by DTT or ADP-sensitive membrane protein thiol oxidation. Taken together, these results provide evidence that mitochondrial permeability transition induced by chemically generated singlet oxygen is mediated by the oxidation of membrane protein thiols.
- Subjects :
- Animals
Cell Respiration drug effects
Electron Transport drug effects
Intracellular Membranes drug effects
Membrane Potentials drug effects
Membrane Proteins metabolism
Mitochondria, Liver ultrastructure
Naphthols pharmacology
Permeability drug effects
Rats
Rats, Wistar
Singlet Oxygen chemistry
Sulfhydryl Compounds metabolism
Mitochondria, Liver drug effects
Singlet Oxygen pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0145-479X
- Volume :
- 34
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of bioenergetics and biomembranes
- Publication Type :
- Academic Journal
- Accession number :
- 12171065
- Full Text :
- https://doi.org/10.1023/a:1016075218162