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Generation and phenotypic analysis of CHIF knockout mice.
- Source :
-
American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2002 Sep; Vol. 283 (3), pp. F569-77. - Publication Year :
- 2002
-
Abstract
- Corticosteroid hormone-induced factor (CHIF) is a short epithelial-specific protein that is independently induced by aldosterone and a high-K(+) diet. It is a member of the FXYD family of single-span transmembrane proteins that include phospholemman, Mat-8, and the gamma-subunit of Na(+)-K(+)-ATPase. A number of studies have suggested that these proteins are involved in the regulation of ion transport and, in particular, functionally interact with the Na(+)-K(+)-ATPase. The present study describes the characterization, targeted disruption, and phenotypic analysis of the mouse CHIF gene. The CHIF knockout mice are viable and not distinguishable from wild-type littermates under normal conditions. Under K(+) loading, they have a twofold higher urine volume and an increased glomerular filtration rate. Similar but smaller effects are observed in mice fed a low-Na(+) diet. Treating K(+)-loaded mice for 10 days with furosemide resulted in lethality in the knockout mice (17 of 39) but not in the wild-type group (1 of 39). The data are consistent with an effect of CHIF on the Na(+)-K(+)-ATPase that is specific to the outer and inner medullary duct, its major expression site.
- Subjects :
- Amino Acid Sequence
Animals
Blood
Blotting, Southern
DNA Restriction Enzymes metabolism
Diet
Diet, Sodium-Restricted
Embryo, Mammalian cytology
Exons
Furosemide pharmacology
Glomerular Filtration Rate
Introns
Kidney Medulla metabolism
Membrane Proteins chemistry
Membrane Proteins deficiency
Membrane Proteins genetics
Mice
Mice, Knockout
Molecular Sequence Data
Nucleic Acid Hybridization
Osmolar Concentration
Potassium administration & dosage
Potassium Chloride administration & dosage
RNA, Messenger analysis
RNA, Messenger chemistry
Sequence Analysis, DNA
Sodium-Potassium-Exchanging ATPase metabolism
Stem Cells metabolism
Transfection
Urine
Membrane Proteins physiology
Phenotype
Subjects
Details
- Language :
- English
- ISSN :
- 1931-857X
- Volume :
- 283
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Renal physiology
- Publication Type :
- Academic Journal
- Accession number :
- 12167609
- Full Text :
- https://doi.org/10.1152/ajprenal.00376.2001