Back to Search
Start Over
Purification and characterization of a corrinoid compound from Chlorella tablets as an algal health food.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2002 Aug 14; Vol. 50 (17), pp. 4994-7. - Publication Year :
- 2002
-
Abstract
- Vitamin B(12) content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 microg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 microg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy.
- Subjects :
- Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Corrinoids
Luminescent Measurements
Magnetic Resonance Spectroscopy
Spectrophotometry, Ultraviolet
Vitamin B 12 chemistry
Vitamin B 12 isolation & purification
Chlorella chemistry
Food, Organic analysis
Porphyrins chemistry
Porphyrins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 50
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12166996
- Full Text :
- https://doi.org/10.1021/jf020345w