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The ontogeny of fatty acid-binding protein in turkey (Meleagridis gallopavo) intestine and yolk sac membrane during embryonic and early posthatch development.
- Source :
-
Poultry science [Poult Sci] 2002 Jul; Vol. 81 (7), pp. 1065-70. - Publication Year :
- 2002
-
Abstract
- Experiments were conducted to confirm the existence and ontogeny of fatty acid binding protein (FABP) in the intestine and yolk sac membrane of turkey poults (Meleagridis gallopavo) during embryonic and early posthatch development. Intestinal (I-) FABP was measured using an immunoblot procedure incorporating anti-chick liver (L-)FABP antisera. FABP activity in both tissues was also confirmed with a ligand-binding assay incorporating 14C-oleic acid. I-FABP did not cross-react with chick L-FABP antisera until hatch, embryonic day 28 (ED 28), after which there was a 39% increase in I-FABP concentration through the first 3 d posthatch (PD 3). FABP concentration calculated on a total intestinal basis (ng/intestine), however, increased 10-fold through PD 6. Specific activity [disintegrations per minute (dpm)/ mg cytosolic protein] was greatest at hatch and decreased slightly thereafter, whereas specific activity of FABP in the yolk sac membrane peaked between ED 16 and ED 19 and then declined. Total yolk sac activity (dpm/yolk sac membrane), however, plateaued at ED 22 before declining to low levels by PD 3, coincident with the period of maximal lipid transfer out of the yolk.
- Subjects :
- Aging
Animals
Carbon Radioisotopes
Carrier Proteins metabolism
Fatty Acid-Binding Proteins
Immunoblotting
Intestines embryology
Intestines growth & development
Oleic Acid metabolism
Organ Size
Yolk Sac embryology
Yolk Sac growth & development
Carrier Proteins analysis
Intestines chemistry
Neoplasm Proteins
Turkeys embryology
Turkeys growth & development
Yolk Sac chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0032-5791
- Volume :
- 81
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 12162346
- Full Text :
- https://doi.org/10.1093/ps/81.7.1065