Back to Search
Start Over
Murine polymorphonuclear neutrophils produce interferon-gamma in response to pulmonary infection with Nocardia asteroides.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 2002 Aug; Vol. 72 (2), pp. 373-81. - Publication Year :
- 2002
-
Abstract
- Nocardia asteroides causes an acute, necrotizing pneumonia characterized by extensive infiltration of polymorphonuclear neutrophils (PMNs) into the lungs. Although PMNs have historically been classified as end-point cells, recent investigations have indicated that PMNs have the ability to secrete cytokines such as interleukin (IL)-4 and IL-12. This study investigated the ability of PMNs to produce cytokines in a murine model of N. asteroides pulmonary infection. Flow cytometric analysis demonstrated the production of interferon-gamma (IFN-gamma), but not IL-4, by PMNs in response to this infection. IFN-gamma production correlated with peak infiltration of PMNs into the lungs. Cell sorting and enzyme-linked immunosorbent assay were used to confirm cytokine production by cells with nuclear morphology characteristic of PMNs. This is the first report of IFN-gamma production by neutrophils in response to an infection in vivo. These results suggest that PMNs play an important role in directing the host toward a T helper cell type 1 phenotypic response in the lungs.
- Subjects :
- Animals
Enzyme-Linked Immunosorbent Assay
Female
Gene Expression
Interferon-gamma genetics
Interleukin-4 analysis
Mice
Mice, Inbred C57BL
Nocardia Infections microbiology
Nocardia Infections pathology
Nocardia asteroides isolation & purification
Pneumonia, Bacterial microbiology
Pneumonia, Bacterial pathology
Specific Pathogen-Free Organisms
Time Factors
Interferon-gamma biosynthesis
Nocardia Infections immunology
Nocardia asteroides immunology
Pneumonia, Bacterial immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 72
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 12149429