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Diagnosis of malaria by detection of plasmodial lactate dehydrogenase with an immunodot enzyme assay.
- Source :
-
Immunological investigations [Immunol Invest] 2002 May; Vol. 31 (2), pp. 93-106. - Publication Year :
- 2002
-
Abstract
- We have previously demonstrated, using polyclonal and monoclonal antibodies, that the lactate dehydrogenase (LDH) of malaria parasites is immunologically distinct from the host enzyme. The polyclonal antibodies, produced against the affinity purified plasmodial LDH (pLDH) in rabbits, showed specificity to LDH of malaria parasites. In the present study, these anti-pLDH polyclonal antibodies were used to develop an immunodiagnostic test (immunodot enzyme assay of plasmodial LDH) based on the detection of parasite LDH in patient blood. The immunodot enzyme assay of plasmodial LDH was evaluated using blood samples from patients with malaria or other infections. Out of 502 microscopically positive malaria blood samples, 497 blood samples showed positive immunodot assays of pLDH while all the 423 microscopically negative cases were found negative by our test. The blood samples from other infections and non-endemic controls were negative by the immunodot enzyme assay of pLDH. This LDH based test was also found negative in blood samples of cured patients 7 days after chloroquine treatment. The test is simple to perform, can be read visually, econimal, highly specific with a sensitivity of approximately 99% and is thus suitable for accurate diagnosis of malaria in field conditions.
- Subjects :
- Animals
Antibodies, Protozoan
Antibody Specificity
Antimalarials therapeutic use
Chloroquine therapeutic use
Humans
Immunoblotting statistics & numerical data
L-Lactate Dehydrogenase immunology
Malaria drug therapy
Malaria parasitology
Plasmodium immunology
Plasmodium knowlesi enzymology
Plasmodium knowlesi immunology
Rabbits
Sensitivity and Specificity
Immunoblotting methods
L-Lactate Dehydrogenase analysis
L-Lactate Dehydrogenase blood
Malaria diagnosis
Malaria enzymology
Plasmodium enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0882-0139
- Volume :
- 31
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Immunological investigations
- Publication Type :
- Academic Journal
- Accession number :
- 12148951
- Full Text :
- https://doi.org/10.1081/imm-120004801