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Helicobacter pylori HspA Heat-shock Protein Gene Cloning, Expression and Immunogenicity.
- Source :
-
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)] 1999; Vol. 31 (3), pp. 264-268. - Publication Year :
- 1999
-
Abstract
- Helicobacter pylori is the causative agent of gastritis and peptic ulcer in human. The heat-shock protein A (HspA)may stimulate the immunoresponse protecting human body against challenge of H.pylori. The gene encoding the structural A subunit of H.pylori heat-shock protein was amplified from H.pylori chromosomal DNA by PCR, and was inserted in the prokaryotic expression vector pET-22b(+), and then was transformed into the BL-21(DE3)E.coli strain to express the HspA recombinant protein. HspA gene was measured to be 354 base pairs, and the recombinant protein gene encoded polypeptides of 118 amino acid residues, corres-ponding to calculated molecular weight of 15 kD. Western blot analysis of HspA recombinant protein was confirmed that it could be specifically recognized by the serum of H.pylori-infected patients, and could also be re-cognized by the serum of immunized Balb/c mice with HspA itself. This result suggests that HspA may be an effective protein vaccine for prevention and treatment of the infection of H.pylori.
Details
- Language :
- English
- ISSN :
- 0582-9879
- Volume :
- 31
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 12136175