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Distribution of neuropeptide Y Y1 receptors in rodent peripheral tissues.
- Source :
-
The Journal of comparative neurology [J Comp Neurol] 2002 Aug 05; Vol. 449 (4), pp. 390-404. - Publication Year :
- 2002
-
Abstract
- Using a sensitive immunohistochemical technique, the localization of neuropeptide Y (NPY) Y1-receptor (Y1R)-like immunoreactivity (LI) was studied in various peripheral tissues of rat. Wild-type (WT) and Y1R-knockout (KO) mice were also analyzed. Y1R-LI was found in small arteries and arterioles in many tissues, with particularly high levels in the thyroid and parathyroid glands. In the thyroid gland, Y1R-LI was seen in blood vessel walls lacking alpha-smooth muscle actin, i.e., perhaps in endothelial cells of capillaries. Larger arteries lacked detectable Y1R-LI. A distinct Y1R-immunoreactive (IR) reticulum was seen in the WT mouse spleen, but not in Y1R-KO mouse or rat. In the gastrointestinal tract, Y1R-positive neurons were observed in the myenteric plexus, and a few enteroendocrine cells were Y1R-IR. Some cells in islets of Langerhans in the pancreas were Y1R-positive, and double immunostaining showed coexistence with somatostatin in D-cells. In the urogenital tract, Y1R-LI was observed in the collecting tubule cells of the renal papillae and in some epithelial cells of the seminal vesicle. Some chromaffin cells of adrenal medulla were positive for Y1R. The problem of the specificity of the Y1R-LI is evaluated using adsorption tests as well as comparisons among rat, WT mouse, and mouse with deleted Y1R. Our findings support many earlier studies based on other methodologies, showing that Y1Rs on smooth muscle cells of blood vessels mediate NPY-induced vasoconstriction in various organs. In addition, Y1Rs in other cells in parenchymal tissues of several organs suggest nonvascular effects of NPY via the Y1R.<br /> (Copyright 2002 Wiley-Liss, Inc.)
- Subjects :
- Animals
Cardiovascular System metabolism
Cardiovascular System ultrastructure
Digestive System blood supply
Digestive System metabolism
Digestive System ultrastructure
Endocrine System blood supply
Endocrine System metabolism
Endocrine System ultrastructure
Female
Ganglia, Autonomic blood supply
Ganglia, Autonomic metabolism
Ganglia, Autonomic ultrastructure
Lymphatic System blood supply
Lymphatic System metabolism
Lymphatic System ultrastructure
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Muscle, Smooth, Vascular ultrastructure
Neurons chemistry
Neurons ultrastructure
Organ Specificity physiology
Rats
Rats, Sprague-Dawley
Receptors, Neuropeptide Y deficiency
Receptors, Neuropeptide Y genetics
Receptors, Neuropeptide Y ultrastructure
Skin blood supply
Skin metabolism
Skin ultrastructure
Trachea blood supply
Trachea metabolism
Trachea ultrastructure
Urogenital System blood supply
Urogenital System metabolism
Urogenital System ultrastructure
Muscle, Smooth, Vascular metabolism
Receptors, Neuropeptide Y metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9967
- Volume :
- 449
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of comparative neurology
- Publication Type :
- Academic Journal
- Accession number :
- 12115674
- Full Text :
- https://doi.org/10.1002/cne.10303