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Sequence Analysis of SOD Gene of Bombyx mori Nuclear Polyhedrosis Virus and Its Expression in E.coli.

Authors :
Wang WB
Ji P
Wu J
He JL
Wu XF
Source :
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)] 1999; Vol. 31 (4), pp. 405-408.
Publication Year :
1999

Abstract

Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress. SOD gene of Bombyx mori nuclear polyhedrosis virus has been cloned by PCR, and is expressed in E.coli with the activity of SOD being 576.13 u/mL. DNA sequence analysis shows that SOD gene of BmNPV encodes 151 amino acids. The homology between BmNPV and AcNPV of the nucleotide sequences of SOD gene is 97.2%, and 56% between BmNPV and human SOD1.

Details

Language :
English
ISSN :
0582-9879
Volume :
31
Issue :
4
Database :
MEDLINE
Journal :
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
Publication Type :
Academic Journal
Accession number :
12114994