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Distribution of estrone sulfatase in rat brain determined by in vitro autoradiography with 16alpha-[18F]fluoroestradiol-3,17beta-disulfamate.
Distribution of estrone sulfatase in rat brain determined by in vitro autoradiography with 16alpha-[18F]fluoroestradiol-3,17beta-disulfamate.
- Source :
-
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine [Appl Radiat Isot] 2002 Jun; Vol. 56 (6), pp. 773-80. - Publication Year :
- 2002
-
Abstract
- 16Alpha-fluoroestradiol-3,17beta-disulfamate (FESDS) strongly inhibits estrone sulfatase (ES), an enzyme which is also present in the brain. The enzyme is probably involved in important regulatory functions of neurosteroids which may be disturbed in certain brain diseases. In the present study, [18F]FESDS was used to measure the amount of ES in various rat brain regions using quantitative in vitro autoradiography. The obtained values vary between 0.29 pmol (mg protein)(-1) (pons) and 11.5 pmol (mg protein)(-1) (striatum). They are positively correlated with the enzyme activity measured in homogenates of the corresponding regions. Because this radiotracer binds also to carbonic anhydrase in the brain it is only of limited use for in vivo imaging studies.
- Subjects :
- Adenocarcinoma
Animals
Autoradiography methods
Breast Neoplasms
Estradiol analogs & derivatives
Female
Humans
Kinetics
Organ Specificity
Rats
Reverse Transcriptase Polymerase Chain Reaction
Sulfatases genetics
Transcription, Genetic
Tumor Cells, Cultured
Brain enzymology
Estradiol pharmacokinetics
Fluorine Radioisotopes
Sulfatases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0969-8043
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 12102332
- Full Text :
- https://doi.org/10.1016/s0969-8043(02)00060-x