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The murine pantothenate kinase (Pank1) gene encodes two differentially regulated pantothenate kinase isozymes.
- Source :
-
Gene [Gene] 2002 May 29; Vol. 291 (1-2), pp. 35-43. - Publication Year :
- 2002
-
Abstract
- Pantothenate kinase (PanK) is a rate-determining enzyme in coenzyme A (CoA) biosynthesis. The mouse murine pantothenate kinase (Pank1) gene consists of seven introns and eight exons and is located on chromosome 19 (19C2-3). Two biochemically distinct PanK1 protein isoforms, PanK1 alpha and PanK1 beta, are encoded by the Pank1 gene. Both proteins have the same 363 amino acid catalytic domain encoded by exons 2 through 7. The PanK1 beta transcript begins with exon 1 beta and translates into a ten-residue amino terminus plus the catalytic domain. The PanK1 alpha transcript initiates at an alternate upstream site at exon 1 alpha which is spliced with exon 2, excluding exon 1 beta. Exon 1 alpha encodes a 184-residue regulatory domain at the amino terminus of the PanK1 alpha protein that confers feedback inhibition by free CoA and long-chain acyl-CoA, and increases the regulation of PanK enzyme activity by acetyl-CoA and malonyl-CoA. Differential expression of the PanK1 alpha and PanK1 beta transcripts would alter the amount of CoA produced in cells as a function of the ratio of free CoA to acetyl-CoA, a reflection of the metabolic status of the tissue.
- Subjects :
- Acetyl Coenzyme A pharmacology
Alternative Splicing
Animals
COS Cells
Cell Line
Chromosome Mapping
Cloning, Molecular
Coenzyme A pharmacology
DNA, Complementary chemistry
DNA, Complementary genetics
Dose-Response Relationship, Drug
Enzyme Inhibitors pharmacology
Exons
Gene Expression Regulation, Enzymologic
Genes genetics
Humans
In Situ Hybridization, Fluorescence
Introns
Isoenzymes drug effects
Isoenzymes genetics
Isoenzymes metabolism
Mice
Molecular Sequence Data
Palmitoyl Coenzyme A pharmacology
Phosphotransferases (Alcohol Group Acceptor) drug effects
Phosphotransferases (Alcohol Group Acceptor) metabolism
Sequence Alignment
Sequence Analysis, DNA
Phosphotransferases (Alcohol Group Acceptor) genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1119
- Volume :
- 291
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 12095677
- Full Text :
- https://doi.org/10.1016/s0378-1119(02)00564-4