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Inhibition of cultured bovine aortic endothelial cell proliferation by sodium spirulan, a new sulfated polysaccharide isolated from Spirulina platensis.
- Source :
-
Planta medica [Planta Med] 2002 Jun; Vol. 68 (6), pp. 505-9. - Publication Year :
- 2002
-
Abstract
- Sodium spirulan (Na-SP) is a sulfated polysaccharide isolated from the blue-green alga Spirulina platensis, which consists of two types of disaccharide repeating units, O-hexuronosyl-rhamnose (aldobiuronic acid) and O-rhamnosyl-3-O-methylrhamnose (acofriose) with sulfate groups, other minor saccharides and sodium ion. Vascular endothelial cells are present on the inner surface of blood vessels in a monolayer and have anticoagulant properties. To address the question whether Na-SP influences the maintenance of endothelial cell monolayers, we investigated the proliferation of cultured bovine aortic endothelial cells treated with Na-SP. It was found that Na-SP has an inhibitory activity on endothelial cell proliferation accompanied with suppression of whole protein synthesis but without non-specific cell damage. The inhibitory activity of Na-SP was the strongest when compared to that of heparan sulfate, heparin, dextran sulfate, dermatan sulfate, chondroitin sulfate A/C and hyaluronan. Furthermore, it was shown that the inhibitory activity of Na-SP disappeared by either desulfation or depolymerization. The present data suggest that Na-SP is a unique sulfated polysaccharide that strongly inhibits vascular endothelial cell proliferation, and the inhibitory activity requires polymerization of sulfated O-rhamnosyl-acofriose repeating units.
- Subjects :
- Animals
Aorta cytology
Cattle
Cell Division drug effects
Cells, Cultured
Chondroitin Sulfates pharmacology
Dermatan Sulfate pharmacology
Dextran Sulfate pharmacology
Dose-Response Relationship, Drug
Endothelium, Vascular cytology
Heparin pharmacology
Heparitin Sulfate pharmacology
Hyaluronic Acid pharmacology
L-Lactate Dehydrogenase metabolism
Leucine metabolism
Leucine pharmacology
Mannose chemistry
Polysaccharides chemistry
Sodium chemistry
Sulfates chemistry
Thymidine metabolism
Thymidine pharmacology
Tritium
Aorta drug effects
Cyanobacteria
Endothelium, Vascular drug effects
Mannose analogs & derivatives
Polysaccharides isolation & purification
Polysaccharides pharmacology
Sulfates isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0943
- Volume :
- 68
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Planta medica
- Publication Type :
- Academic Journal
- Accession number :
- 12094292
- Full Text :
- https://doi.org/10.1055/s-2002-32564