An enzymatic fluorometric assay for pyridoxal with high specificity and sensitivity.

Authors :: Trongpanich Y
Mito M
Yagi T
Source :: Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2002 May; Vol. 66 (5), pp. 1152-4.
Publication Year :: 2002
Abstract: An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol: the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography.

Subjects :: Chromatography, High Pressure Liquid
Sensitivity and Specificity
Alcohol Oxidoreductases chemistry
Pyridoxal analysis
Spectrometry, Fluorescence methods

Full Text Access

Tools