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Cloning and sequencing of the serine dehydrogenase gene from Agrobacterium tumefaciens.

Authors :
Fujisawa H
Nagata S
Chowdhury EK
Matsumoto M
Misono H
Source :
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2002 May; Vol. 66 (5), pp. 1137-9.
Publication Year :
2002

Abstract

The structural gene for NADP+-dependent serine dehydrogenase [EC 1.1.1.-] from Agrobacterium tumefaciens ICR 1600 was cloned into Escherichia coli cells and its complete DNA sequence was analyzed. The gene encodes a polypeptide containing 249 amino acid residues. The enzyme had high sequence similarity to short-chain alcohol dehydrogenases from bacteria and unknown proteins of Haemophilus influenzae, Escherichia coli, and Saccharomyces cerevisiae.

Details

Language :
English
ISSN :
0916-8451
Volume :
66
Issue :
5
Database :
MEDLINE
Journal :
Bioscience, biotechnology, and biochemistry
Publication Type :
Academic Journal
Accession number :
12092831
Full Text :
https://doi.org/10.1271/bbb.66.1137