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Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2002 Jul; Vol. 40 (7), pp. 2659-61. - Publication Year :
- 2002
-
Abstract
- One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.
- Subjects :
- Bacteremia diagnosis
Bacteriological Techniques methods
Bacteriological Techniques statistics & numerical data
Base Sequence
DNA Primers genetics
Humans
Methicillin Resistance genetics
Penicillin-Binding Proteins
Polymerase Chain Reaction statistics & numerical data
Sensitivity and Specificity
Staphylococcal Infections diagnosis
Staphylococcus aureus drug effects
Bacterial Proteins
Carrier Proteins genetics
Genes, Bacterial
Hexosyltransferases
Muramoylpentapeptide Carboxypeptidase genetics
Peptidyl Transferases
Polymerase Chain Reaction methods
Staphylococcus aureus genetics
Staphylococcus aureus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 40
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12089301
- Full Text :
- https://doi.org/10.1128/JCM.40.7.2659-2661.2002