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Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2002 Jun 15; Vol. 185 (12), pp. 1709-16. Date of Electronic Publication: 2002 May 31. - Publication Year :
- 2002
-
Abstract
- Antigen-specific, cytokine flow cytometry was used to analyze the prevalence and frequency of CD4 and CD8 memory T cells specific for the abundantly expressed cytomegalovirus (CMV) phosphoprotein 65 (pp65) in healthy CMV IgG-seropositive individuals. Stimulation of peripheral blood mononuclear cells with peptide pools and individual peptides derived from the pp65 amino acid sequence in 40 donors revealed that 63% of donors had a detectable CD4 T cell response and that 83% of donors had a detectable CD8 T cell response against this protein. The overall frequencies of T cells directed against pp65 were analyzed for 20 donors by stimulation with peptide pools covering the complete pp65 protein and were as high as 2 in 1000 and 9 in 1000 (median) peripheral blood CD4 and CD8 T cells, respectively. In addition, a comparison between CD4 responses to a CMV lysate containing various CMV proteins and pp65-specific responses in 9 donors indicated that pp65 was a dominant target of the CMV-specific CD4 T cell response in some, but not all, donors. Several new T cell epitopes were identified.
- Subjects :
- Amino Acid Sequence
CD4-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Epitopes, T-Lymphocyte immunology
Humans
Molecular Sequence Data
CD4-Positive T-Lymphocytes virology
CD8-Positive T-Lymphocytes virology
Cytomegalovirus immunology
Phosphoproteins immunology
Viral Matrix Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 185
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 12085315
- Full Text :
- https://doi.org/10.1086/340637