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Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci.
- Source :
-
Journal of cell science [J Cell Sci] 2002 Jul 15; Vol. 115 (Pt 14), pp. 2839-46. - Publication Year :
- 2002
-
Abstract
- The standard rye cultivar 'Imperial' and a structural variant carrying an intact 1R chromosome and two telocentric 1R chromosomes (short and long arms) were used to investigate expression patterns of homologous rDNA loci, and the influence of chromosome structural change on their interphase organisation and relative disposition. Sequential silver staining and in situ hybridization with the rDNA probe pTa71, established a correspondence between the expression and organization patterns of rDNA domains in metaphase and interphase cells. In most cells of the cultivar Imperial, nucleolar organizer region (NOR) silver staining on metaphase chromosomes with equivalent numbers of rDNA genes revealed a size heteromorphism between homologous rDNA loci, resulting from their differential expression. NOR heteromorphism in the structural variant line was significantly reduced. The preferential activity of one NOR over its homologue was found to be random within cells and independent of parental origin. Nucleotypic modifications mediated by changes in the 1R chromosome structure include increased proximity between homologous rDNA loci in interphase, and an increase in the frequency of cells with intra-nucleolar ribosomal condensed chromatin. These results seem to indicate a 'sequence recognition' process for the regulation of homologous loci.
- Subjects :
- Cell Cycle drug effects
Cell Nucleolus drug effects
Cell Nucleolus genetics
Chromosome Mapping
Chromosomes drug effects
DNA, Ribosomal drug effects
Gene Expression Regulation, Plant drug effects
Meristem cytology
Meristem drug effects
Meristem genetics
Metaphase drug effects
Metaphase genetics
Mitosis drug effects
Mitosis genetics
Nucleic Acid Synthesis Inhibitors pharmacology
Nucleolus Organizer Region drug effects
RNA genetics
Secale cytology
Secale metabolism
Cell Cycle genetics
Chromosomes genetics
DNA, Ribosomal genetics
Gene Expression Regulation, Plant genetics
Genome, Plant
Nucleolus Organizer Region genetics
Secale genetics
Sequence Homology, Nucleic Acid
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9533
- Volume :
- 115
- Issue :
- Pt 14
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 12082145
- Full Text :
- https://doi.org/10.1242/jcs.115.14.2839