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Antigen-dependent release of IFN-gamma by cytotoxic T cells up-regulates Fas on target cells and facilitates exocytosis-independent specific target cell lysis.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2002 Jul 01; Vol. 169 (1), pp. 145-50. - Publication Year :
- 2002
-
Abstract
- Effector cytolytic T (Tc) lymphocytes, deficient in the exocytosis-mediated pathway of target cell lysis, induce Fas on target cells and, in turn, delayed cell death and apoptosis via the Fas ligand-Fas interaction. The induction of Fas can be blocked by anti- IFN-gamma Abs. This Fas up-regulation on initially Fas-negative target cells is not mediated by TCR-MHC/peptide signaling per se, but by secreted IFN-gamma from Tc cells after Ag engagement. The Fas up-regulation by Tc cells can be mimicked by treatment of target cells with rIFN-gamma. Tc cells from IFN-gamma knockout mice do not induce Fas expression on target cells. Tc cell-mediated Fas expression on third party, bystander, target cells does not enhance their susceptibility to lysis by these nominal effector cells. The results are discussed as to the possible relevance of the phenomenon in efficiency and regulation of the Tc cell response to infections by viruses.
- Subjects :
- Animals
Antibodies, Monoclonal pharmacology
Bystander Effect immunology
Chromium Radioisotopes metabolism
Cytotoxicity Tests, Immunologic methods
DNA antagonists & inhibitors
DNA Fragmentation genetics
DNA Fragmentation immunology
Exocytosis genetics
Interferon-gamma deficiency
Interferon-gamma immunology
Interferon-gamma pharmacology
L Cells
Leukemia L1210
Membrane Glycoproteins deficiency
Membrane Glycoproteins genetics
Mice
Mice, Inbred AKR
Mice, Inbred C57BL
Mice, Knockout
Perforin
Pore Forming Cytotoxic Proteins
Recombinant Proteins pharmacology
Serine Endopeptidases deficiency
Serine Endopeptidases genetics
Solubility
T-Lymphocytes, Cytotoxic immunology
Up-Regulation genetics
fas Receptor physiology
Cytotoxicity, Immunologic genetics
Epitopes, T-Lymphocyte immunology
Exocytosis immunology
H-2 Antigens physiology
Interferon-gamma metabolism
T-Lymphocytes, Cytotoxic metabolism
Up-Regulation immunology
fas Receptor biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 169
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 12077239
- Full Text :
- https://doi.org/10.4049/jimmunol.169.1.145