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Phenotypic and genotypic characterisation of blood isolates of coagulase-negative staphylococci in the newborn.
- Source :
-
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2002 Apr; Vol. 110 (4), pp. 332-9. - Publication Year :
- 2002
-
Abstract
- Coagulase-negative staphylococci (CNS) are the leading cause of late-onset sepsis in newborns (>72 h of age). Our aim was to determine whether phenotypic and/or genotypic differences existed between blood isolates of CNS regarded as inducers of sepsis or as contaminants. Ninety-seven bloodisolates of CNS recovered from newborns at the neonatal intensive care unit, Orebro, Sweden in 1983-1997 were analysed. Twenty-nine of them (30%) were classified as sepsis isolates and 68 (70%) as contaminants. The most prevalent species was Staphylococcus epidermidis (n=59). Staphylococcus haemolyticus (n=16) was most often isolated from newborns with the lowest gestational age and birth weight. Biochemical typing using the Phene Plate system (PhP) and genotyping using pulsed-field gel electrophoresis (PFGE) showed that the S. epidermidis isolates regarded as inducers of sepsis (n=16) were more homogeneous than isolates considered contaminants (n=37). One main genotypic group, representing seven (44%) isolates, was identified among the sepsis isolates. Phenotypically the S. epidermidis sepsis isolates comprised three major clusters. In contrast, among the S. epidermidis contaminants, eight genotypic groups and two phenotypic clusters were identified. The dominating genotypic group among the sepsis isolates of S. epidermidis may represent strains with higher invasive capacity.
- Subjects :
- Bacterial Typing Techniques
Coagulase genetics
Genotype
Humans
Infant, Newborn
Sepsis blood
Staphylococcus classification
Staphylococcus genetics
Staphylococcus pathogenicity
Virulence genetics
Coagulase deficiency
Sepsis microbiology
Staphylococcus enzymology
Staphylococcus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0903-4641
- Volume :
- 110
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 12076269
- Full Text :
- https://doi.org/10.1034/j.1600-0463.2002.100408.x