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Cardiac gene expression profile and lipid accumulation in response to starvation.
- Source :
-
American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2002 Jul; Vol. 283 (1), pp. E94-E102. - Publication Year :
- 2002
-
Abstract
- Starvation induces many biochemical and histological changes in the heart; however, the molecular events underlying these changes have not been fully elucidated. To explore the molecular response of the heart to starvation, microarray analysis was performed together with biochemical and histological investigations. Serum free fatty acids increased twofold in both 16- and 48-h-fasted mice, and cardiac triglyceride content increased threefold and sixfold in 16- and 48-h-fasted mice, respectively. Electron microscopy showed numerous lipid droplets in hearts of 48-h-fasted mice, whereas fewer numbers of droplets were seen in hearts from 16-h-fasted mice. Expression of 11,000 cardiac genes was screened by microarrays. More than 50 and 150 known genes were detected by differential expression analysis after 16- and 48-h-fasts, respectively. Genes for fatty acid oxidation and gluconeogenesis were increased, and genes for glycolysis were decreased. Many other genes for metabolism, signaling/cell cycle, cytoskeleton, and tissue antigens were affected by fasting. These data provide a broad perspective of the molecular events occurring physiologically in the heart in response to starvation.
- Subjects :
- Animals
Blotting, Northern
Body Weight physiology
Cell Cycle genetics
Cytoskeleton genetics
Energy Metabolism genetics
Fatty Acids metabolism
Female
Gluconeogenesis genetics
Glycolysis genetics
Lipids blood
Male
Mice
Mice, Inbred C57BL
Myocardium cytology
Myocardium ultrastructure
Oligonucleotide Array Sequence Analysis
Oxidation-Reduction
Signal Transduction genetics
Gene Expression Profiling
Lipid Metabolism
Myocardium metabolism
Starvation metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0193-1849
- Volume :
- 283
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Endocrinology and metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 12067848
- Full Text :
- https://doi.org/10.1152/ajpendo.00017.2002