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A new lipophilic fluorescent probe for interaction studies of bioactive lipopeptides with membrane models.
- Source :
-
Bioorganic & medicinal chemistry letters [Bioorg Med Chem Lett] 2002 Jul 08; Vol. 12 (13), pp. 1731-4. - Publication Year :
- 2002
-
Abstract
- The new fluorescent lipophilic moiety 11-[(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-acetyl)amino]undecanoic acid (AMCA-omegaAud-OH) was introduced by SPPS at the N-terminus of the immunodominant epitope GpMBP(74-85). FRET experiments using the new fluorescent lipopeptide demonstrate that the peptide interacts with much more affinity with the membrane compared to the lipid free analogue.
- Subjects :
- Animals
Binding Sites
Energy Transfer
Immunodominant Epitopes chemistry
Immunodominant Epitopes immunology
Lipid Bilayers chemistry
Lipoproteins immunology
Lipoproteins metabolism
Membrane Lipids chemistry
Membrane Lipids metabolism
Models, Molecular
Palmitic Acid chemistry
Palmitic Acid metabolism
Rats
Spectrometry, Fluorescence
Spectrometry, Mass, Electrospray Ionization
Fluorescent Dyes chemical synthesis
Fluorescent Dyes chemistry
Lipoproteins chemistry
Membranes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0960-894X
- Volume :
- 12
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Bioorganic & medicinal chemistry letters
- Publication Type :
- Academic Journal
- Accession number :
- 12067548
- Full Text :
- https://doi.org/10.1016/s0960-894x(02)00253-6