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Genetic analysis of factor V Leiden in a family with history of thrombosis and venous leg ulcers.
- Source :
-
Journal of cellular and molecular medicine [J Cell Mol Med] 2000 Oct-Dec; Vol. 4 (4), pp. 297-302. - Publication Year :
- 2000
-
Abstract
- Inherited resistance to activated protein C has been recognized as a major risk factor for thrombosis. The factor V Leiden mutation, which is detectable by molecular DNA techniques, is responsible for 95% of cases of activated protein C resistance. In our study one patient with venous leg ulcers from a family with a history of thrombosis showed factor V Leiden mutation. Genotypic analysis demonstrated that the patient was homozygous for factor V Leiden. All family members of the index subject showed the same abnormalities. Two were homozygous and 3 were heterozygous for factor V Leiden mutation. The polymerase chain reaction was used to amplify exon 10 of the factor V gene, followed by enzymatic digestion with MnlI for mutation detection. Patients with a family history of thrombosis and factor V Leiden have an increased risk of venous leg ulcers. Screening for factor V Leiden may be indicated in patients with venous leg ulcers and their family members.
Details
- Language :
- English
- ISSN :
- 1582-4934
- Volume :
- 4
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of cellular and molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 12067465
- Full Text :
- https://doi.org/10.1111/j.1582-4934.2000.tb00130.x