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Platelet-activating factor and lyso-PAF possess direct antimicrobial properties in vitro.
- Source :
-
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2002 Feb; Vol. 110 (2), pp. 158-64. - Publication Year :
- 2002
-
Abstract
- The effects of platelet-activating factor (PAF) and lyso-platelet-activating factor (L-PAF) at concentrations of 0.25-20 microg/ml on potassium transport and growth of gram-positive and gram-negative bacteria have been investigated in vitro and compared with those of lysophosphatidylcholine (LPC). Potassium transport was determined using 86Rb+ as tracer, while growth was measured according to the extent of uptake of radiolabeled amino acids. All of the test phospholipids caused dose-related inhibition of 86Rb+-uptake and growth of gram-positive bacteria, the order of potency being PAF>LPC>L-PAF. Gram-negative bacteria, on the other hand, were less sensitive to the inhibitory effects of the phospholipids on K+ transport and growth. Some, but not all, of the gram-positive and gram-negative bacteria were able to degrade LPC, but not PAF or L-PAF, demonstrating that enzymatic degradation of phospholipids does not explain the differential sensitivity to these agents. The bioactive phospholipids LPC, PAF and L-PAF may represent an oxygen-independent antimicrobial host defense system operative primarily against gram-positive bacteria.
- Subjects :
- Gram-Negative Bacteria growth & development
Gram-Negative Bacteria metabolism
Gram-Positive Bacteria growth & development
Gram-Positive Bacteria metabolism
Lysophosphatidylcholines pharmacology
Potassium metabolism
Rubidium metabolism
Anti-Bacterial Agents pharmacology
Gram-Negative Bacteria drug effects
Gram-Positive Bacteria drug effects
Inflammation Mediators pharmacology
Platelet Activating Factor analogs & derivatives
Platelet Activating Factor pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0903-4641
- Volume :
- 110
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 12064871
- Full Text :
- https://doi.org/10.1034/j.1600-0463.2002.100206.x