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Reversible and irreversible components of [(3)H]-N-propylnorapomorphine interaction with rat striatal membranes.
- Source :
-
Neuroscience letters [Neurosci Lett] 2002 Jun 07; Vol. 325 (2), pp. 111-4. - Publication Year :
- 2002
-
Abstract
- The kinetics of L-(-)-[N-propyl-(3)H(N)]-norapomorphine ([(3)H]NPA) interactions with rat striatal membranes were studied. The analysis revealed that in addition to specific dopaminergic binding a substantial part of the radioligand was bound irreversibly to heterogeneous populations of non-specific binding sites of these membranes. The specific binding of [(3)H]NPA with dopamine receptors, determined from the differences of kinetic curves of total and non-specific binding, was fast, reversible, and revealed high affinity. The irreversible component was heterogeneous and seems to be related to oxidative degradation of the radioligand, as the rate of this process was substantially reduced by antioxidants like ascorbic acid and dithiothreitol.
- Subjects :
- Animals
Antioxidants pharmacology
Ascorbic Acid pharmacology
Binding Sites
Binding, Competitive
Cell Membrane metabolism
Dithiothreitol pharmacology
Dopamine metabolism
Kinetics
Oxidation-Reduction drug effects
Radioactive Tracers
Rats
Receptors, Dopamine metabolism
Tritium
Apomorphine analogs & derivatives
Apomorphine metabolism
Neostriatum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 325
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 12044634
- Full Text :
- https://doi.org/10.1016/s0304-3940(02)00250-1